Use of magnetic beads in Next-Generation Sequencing in the Construction of Low Cost 16S rRNA Gene Genomic Libraries

Maria do Carmo Catanho Pereira de Lyra, Douglas Alfradique Monteiro, Pedro Sodré do Rêgo Barros, Marcos da Silveira Regueira Neto, Heide Lacerda Alves da Cruz, Leandro Reis Costa Santos, Augusto Cesar de Arruda Santana, Valdir Queiroz Balbino, Ana Dolores Santiago de Freitas, Caio Tavora Coelho da Costa Rachid

Resumo


This work aimed to test the new Mag-Bind® TotalPure magnetic beads in the construction of a genomic library of corn and sugarcane endophytes. The technique Next-Generation Sequencing (NGS) is very expensive and for that, one of the most expensive reagents are the magnetics beads that serve to purify the amplified DNA and separate by size depending on their proportion, as shown in the Illumina protocol. We observed that the use of these beads, which are five times cheaper and have the same capacity and quality as those on the market, are good for a satisfactory study of metagenomics and microbiomes. We conclude that the use of omega brand beads has an excellent result in the construction of a genomic library for NGS.


Palavras-chave


Metagenomics technique, NGS, 16S Amplicon PCR, bioinformatics

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Referências


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DOI: https://doi.org/10.12661/pap.2022.004

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Pesquisa Agropecuária Pernambucana
ISSN 0100-8501 (impresso)
ISSN 2446-8053 (online)


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